| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Minnesota Rush Minnesota Rush - B09 Grey |
0101-07UB09-0058 | Chris Graham |
| A2 | Minnesota Rush Minnesota Rush - B09 Black |
0101-07UB09-0060 | Fio Dos Santos |
| A3 | New Prague Soccer Club New Prague Soccer Club - B09 |
0101-08UB09-0037 | Derek Sutherland |
| A4 | Owatonna Soccer FC Owatonna B9 Palmer |
0101-11UB09-0016 | Nathan Palmer |
| A5 | Owatonna Soccer FC Owatonna B9 Gendron |
0101-11UB09-0017 | Nate Gendron |
| A6 | Skills FC Skills FC - B09 |
0101-27UB09-0004 | Jason Popkin |
| B1 | Minnesota Rush Albert Lea- MN Rush - B09- 01 |
0101-07UB09-0081 | Jens Levisen |
| B2 | Minnesota Rush Albert Lea- MN Rush - B09- 02 |
0101-07UB09-0082 | Corbyn Olson |
| B3 | Owatonna Soccer FC Owatonna B9 Fox |
0101-11UB09-0047 | Landon Fox |
| B4 | Waseca Soccer Club Waseca Soccer Club |
0101-22UB09-0004 | Ned Barbknecht |
| B5 | New Ulm United Soccer Club New Ulm United Soccer Club - B09 |
0101-09UB09-0035 | Paul Huebner |
| B6 | Mankato United Soccer Club Mankato Unit - B09 |
0101-05BB09-0007 | Shannon Doehling |
| B7 | Tri-City United Soccer Association Tri-City United Soccer Association - B09 White |
0101-14UB09-0023 | Erik Sloboden |
| C1 | Byron Youth Soccer Association Byron Youth - B09 |
0101-02UB09-0005 | Robb Rice |
| C2 | Rush WI West Soccer Club Rush WI West - B09 Blue |
0101-24UB09-0002 | Ryan Lewis |
| C3 | Rush WI West Soccer Club Rush WI West - B09 White |
0101-24UB09-0003 | Chuye Yang |
| C4 | Minnesota Rush Minnesota Rush - B09 Blue |
0101-07UB09-0059 | Eduardo Kunioshi |
| C5 | Minnesota Rush Minnesota Rush - B09 White |
0101-07UB09-0061 | Vinicius Pereira |
| C6 | Minnesota Rush Austin MN Rush - B09 |
0101-07UB09-0097 | Fabian Becerril |